Modern cardiovascular research requires a multidisciplinary approach. A number of the resources and methods used are best provided by core facilities that can share their expertise and scientific tools with a wide variety of laboratories. The MCRI has built a series of such Core Facilities over the past decade that provide research scientists in the MCRI and throughout the Tufts Health Sciences Campus with access to state-of-the-art approaches, ranging from viral vectors to novel human cell lines.
The Molecular Cardiology Research Institute is invested in developing and maintaining an infrastructure that provides investigators with access to state-of-the-art core facilities, dedicated to basic and translational research. Active MCRI core facilities include: Mouse Physiology, Transgenic/ES cell and Cell Culture. A Typhoon Wave Imager is also available.
The MCRI Mouse Physiology Facility is dedicated to the application of whole-animal mouse models to pursue questions related to the pathophysiology of cardiovascular disease. This facility includes the equipment necessary to perform continuous ambulatory telemetered EKG monitoring, hemodynamic monitoring and invasive electrophysiological testing. The facility is directed by Mr. Mark Aronovitz, Assistant Professor of Medicine, Tufts University School of Medicine and a full-time member of the MCRI. Mr. Aronovitz is a highly experienced animal scientist with special expertise in animal physiology models.
The MCRI Transgenic Facility has the capability to produce both standard transgenic mice by pronuclear microinjection and knockout/knockin mice by blastocyst injection. Contained within the facility is dedicated animal housing, a microinjection lab, a molecular biology lab, and a tissue culture room, along with a cold room, conference room, and office space. The animal room has a capacity of ~500 state-of-the-art self-ventilated cages (3,500 mice) to house stud and vasectomized males and donor and recipient female mice used in the production of transgenic and knockout/knockin mice. DLAM oversees the care and maintenance of animals, fully in accordance with NIH and institutional guidelines.
The MCRI Cell Culture Core Facility provides harvesting, characterization and maintenance of cultured cell lines to investigators. Storage, thawing and culture of frozen cell lines is available in the facility. The facility has created over 150 cell lines from human blood vessels, both primary and immortalized. Ms. Wendy Baur directs the facility.
The MCRI Histology Core Facility has merged with the Tufts Histology Core managed by the Division of Laboratory Animal Medicine. It contains all equipment necessary for mouse tissue processing, mounting, sectioning and staining. Experienced histologists perform in situ hybridization studies and immunohistochemistry as requested by investigators.
A Typhoon Model 9410 Variable Mode Imager is located within the MCRI and is capable of performing high resolution, high sensitivity imaging and analysis of samples ranging from radiolabeled proteins on SDS-PAGE gels or nucleic acids on Southern or Northern blots, to immunoblots, tissue sections, macro and microarrays.
A Phillips-Sonos 7500 echocardiography System equipped with 12MHz sector array and 10-15MHz linear array transducers dedicated only to research applications to perform high throughput, mouse echocardiography studies, is available. Click here to schedule to use the echocardiography system.
A Quantitative Real Time PCR Machine is located within the MCRI Center for Translational Genomics and is capable of performing quantitative measurement of transcript abundance and genotype. Both TaqMan and SYBR Green assays can be performed on the Eppendorf Mastercycler ep realplex machine. Click here to schedule to use the quantitative real time PCR machine.
The MCRI Adenoviral Core Facility is no longer in operation.