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Blastocyst Microinjection Services
Embryonic stem cell clones to be provided by the user will be microinjected into a minimum of 40 C57B1/6 X B6D2F1 blastocysts per session.
Prior to injection of ES cells into blastocysts, investigators are required to:
1. Fill out an application form. The application should be downloaded, completed, and faxed to 617-636-8362, or delivered to the Facility which is located on the 1st floor of the Tupper Research Institute at 15 Kneeland Street.
2. Provide the TCF with ES cell clones. The ES cells should be mailed/delivered to the TCF when the investigator has been notified of a start date. The TCF recommends maintaining the storage method of cells obtained from an external source, the same as they were received.
PLEASE NOTE: ES cells from sources other than the TCF must undergo Mycoplasma (pulmonis), mouse hepatitis virus and Sendai testing. The TCF recommends sending cells to Charles River Laboratories (1-800-338-9680), as they guarantee a 10 day turnaround and charge a nominal fee. Contact the TCF Director at jlem@tuftsmedicalcenter.org for more information.
The TCF will nurture chimeric mice up to 21 postnatal days, at which point animals will be transferred to the investigator, who will be responsible for breeding and establishing the line of mice. Offsite investigators will arrange the transfer with the TCF in conjunction with their animal facility.
Cost
Tufts Medical Center Investigators: $2,650 *
Tufts Investigators: $4,000 *
External Academic Non-Profit Investigators: $4,000 **
Industry: Contact the TCF for pricing.
*Price excludes the purchase of female donors and per diem expenses, which are charged directly to the
investigator by DLAM. Contact the Core for an estimate of the additional charge.
**Price excludes the purchase of female donors and per diem expenses. The actual amount incurred by the TCF for these purchases will be added to the final bill. Contact the Core for an estimate of the additional charge.
Note: The following group is eligible for a cost subsidy:
MCRI Investigators
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