MCRI Center for Translational Genomics
The MCRI Center for Translational Genomics investigates genetic and biological mechanisms of heart and cardiovascular disease using a two pronged approach. The first approach seeks to identify genes identified that contribute to human cardiovascular disease through large scale genomic screens. The second approach uses molecular and cellular biology techniques complemented by the use of animal models to study genetic regulators of muscle development and their role in muscle disease. The convergence of the two approaches offers an exciting platform for discovering important biological mechanisms and genetic human variations that contribute to disease. IDENTIFYING GENES ASSOCIATED WITH HUMAN DISEASE: Dr. Huggins believes firmly in the benefit of making primary discoveries of human disease whenever possible in humans. Within the past one to two decades the human genome sequence has been completed and a map of genetic markers suitable for studying disease associations has been established. Both candidate gene and genome-wide association studies are performed in the Huggins laboratory. A particular focus is the identification of genetic contributors to bicuspid aortic valve, hypertrophic Cardiomyopathy, dilated Cardiomyopathy as well as metabolic factors including lipoproteins and inflammatory markers. Recently, we are exploring the use of next-generation sequencing-based exome and transcriptome analysis for the comprehensive identification of rare and common coding variants associated with heart muscle disease. These large-scale genetic screens are supported by active collection of DNA and serum samples from patients of the Tufts Medical Center Cardiovascular Center. ANALYSIS OF MUSCLE DEVELOPMENT AND DISEASE: Causative factors of heart and skeletal muscle disease include environmental insults such as ischemia/infarction, toxins, drug exposure and atypical genetic changes. One of the characteristic features of the diseased heart muscle is a reversion of gene expression to a pattern which was present in the developing embryo called the “fetal gene program”. This event affirms an interesting role for developmental regulators and gene transcription in diseased muscle. An active project in the Huggins laboratory is the analysis of how alcohol exposure leads to muscle damage through complex genetic and cellular mechanisms. As we explore the effects of alcohol on muscle precursor cells we hypothesize that one component contributing to alcohol-induced muscle disease involves effects on regeneration- an avenue we are interested in exploring. CALCIFIC AORTIC VALVE DISEASE: Finally, Dr. Huggins has identified through genotype association study approaches genes that contribute to bicuspid aortic valve disease and calcific aortic valve disease. Current work is determining the role of pathway genes that contribute to bone formation in calcific aortic valve disease.
Research Administrator: Dionne Bradford
Gordon Huggins, M.D.
Tufts Medical Center
800 Washington Street, Box 8486
Boston, MA 02111
1. Return of Genetic Results in the Familial Dilated Cardiomyopathy Research Project. J Genet Couns. 2012 Aug 11. [Epub ahead of print].
2. Biogeographic Ancestry, Self-Identified Race, and Admixture-Phenotype Associations in the Heart SCORE Study. Am J Epidemiol. 2012 Jul 6. [Epub ahead of print].
3. Large-scale gene-centric meta-analysis across 39 studies identifies type 2 diabetes loci. Am J Hum Genet. 2012 Mar 9;90(3):410-25. Epub 2012 Feb 9.
4. Obesity susceptibility loci and dietary intake in the Look AHEAD Trial. Am J Clin Nutr. 2012 Apr 18. [Epub ahead of print].
5. Association of Type 2 Diabetes Susceptibility Loci with One-Year Weight 1 Loss in the Look AHEAD Clinical Trial. Obesity, 2012 (in press).
6. Evaluation of New and Established Age-Related Macular Degeneration Susceptibility Genes in the Women's Health Initiative Sight Exam (WHI-SE) Study. Am J Ophthalmol. 2011 Dec;152(6):1005-1013.e1.
7. ANGPTL4 variants E40K and T266M are associated with lower fasting triglyceride levels in Non-Hispanic White Americans from the Look AHEAD Clinical Trial. BMC Med Genet. 2011 Jun 29;12(1):89.
8. Mind the dbGAP: The Application of Data Mining to Identify Biological Mechanisms. Mol Interv. 2011 Apr;11(2):95-102.
9. Lack of association of Klotho gene variants with valvular and vascular calcification in Caucasians: a candidate gene study of the Framingham Offspring Cohort. Nephrol Dial Transplant. 2011 May 12. [Epub ahead of print].
10. Heterogeneity of the phenotypic definition of coronary artery disease and its impact on genetic association studies. Circ Cardiovasc Genet. 2011 Feb 1;4(1):58-67.
See All MCRI Publications